Biosoft PrimerPlex v2.11.21103 PCR或測序引物以及雜交探針的設計評估軟體 英文破解版






破解說明:安裝完成後，請將光碟\crack目錄下的patcher，複製到主程式的
安裝目錄內中，並覆蓋!
"(install)\http\Dwww.premierbiosoft.com\P80\DMIUpdater\DMP2\DM210"
fromcmdpromptrunjava-jarpatcher.jar



軟體簡介:



BiosoftPrimerPlexv2.11.21103PCR或測序引物以及雜交探針的設計評估軟體英文破解版










官方網站：http://www.premierbiosoft.com/primerdesign/index.html

由加拿大的Premier公司開發的專業用於PCR或測序引物以及雜交探針的設計，評估的軟體，
其主要介面是分為序列編輯視窗（Genetank），引物設計視窗（PrimerDesign），瑂切分
析視窗（RestrictionSites）和紋基分析視窗（Motif）。

PrimerPremier'ssearchalgorithmfindsoptimalPCR,multiplex,SNPgenotyping
anddegenerateprimerswiththemostaccuratemeltingtemperatureusingthe
nearestneighborthermodynamicalgorithm.Primersarescreenedforsecondary
structures,dimers,hairpins,homologiesandphysicalpropertiesbeforereporting
thebestonesforyoursequence,inrankedorder.Equippedwithahandycalculator,
youcaneasilymanipulatesequencesandanalyzetheresultsofyourprimerdesign.

LoadthegeneofinterestfromNCBI,selectasearchrange,sitbackandlet
PrimerPremierpickthebestpossibleprimersforyou.

PrimerDesignforSNPGenotypingAssays
WithPrimerPremier,youcanloadsequencesfromdbSNPandhavetheprimersdesigned
flankingtheSNPselected.HundredsofunpublishedSNPscanalsobeloadedby
specifyingthemasvariationfeaturesinstandardGenBank/dbSNPfiles.Afterspecifying
theSNPs,primerscanbedesignedtoamplifythemfordetectionusingaprobe-based
chemistry.

MultiplexPrimerDesign
Foramultiplexexperiment,PrimerPremierenablesyoutodesignmultiplexprimersby
launchingaprimersearchinbatchmodeandthencheckingthecrossreactivityofthe
primersdesigned.PrimerPremierchecksforallthepossiblereactivityanddisplaysthe
moststablestructureformedbyeacholigo.Thisfunctionalityreducesfalseprimingand
ensuresastrongsignalstrength.

AutomaticHomology&TemplateStructureAvoidance
PrimerPremierautomaticallyinterpretstheBLASTsearchresultsandavoidsthoseregions
todesignprimersthathavesignificantcrosshomologieswiththedatabase.Thesehomologous
regionsarehighlightedinthesequenceviewandareavoidedduringprimersearch.

Primerextensionmaybehinderedduetothepresenceoftemplatestructuresatextension
temperature.Toavoidallsuchregions,wherethetemplatemayfolduponitself,theprogram
utilizesaproprietaryalgorithmtocheckforpossiblesecondarystructureswithinthe
templateatafoldingtemperatureyouspecify.Theregionsinvolvedintheformationofa
secondarystructureareunderlinedinthesequenceviewandareavoidedwhiledesigning
primers.

Avoidinghomologousregionsmakestheoligoshighlyspecificandavoidingtemplatestructures
improvestheefficiencyofthedesignedprimers.